University of Missouri: Thesis Student 3D Prints Collagen to Create Tissue Engineering Scaffolds3/22/2019 University of Missouri: Thesis Student 3D Prints Collagen to Create Tissue Engineering Scaffolds https://ift.tt/2Hz9v7t University of Missouri thesis student, Christopher John Glover, explores the use of 3D printed structures in bioprinting, outlining his findings further in ‘In Situ Polymerizing Collagen for the Development of 3D Printed Tissue Engineering Scaffolds.’ Extolling the virtues of collagen while also discussing challenges in using it, Glover explains that this natural material has been a favorite in tissue engineering, demonstrating excellent protein structures for ventures in the lab.
There can be difficulty in using collagen for some types of tissue regeneration though, and other disadvantages such as the amount of time it takes to progress from a gelatinous state to a solid. For this project, Glover studied the manufacturing of 3D collagen-based scaffolds which he enhanced with a variety of anti-inflammatory agents such as gold nanoparticles and curcumin. Specifically, he used in situ polymerizing collagen (IPC), a unique material derived from Type 1 porcine collagen. In experimenting, he performed different post printing treatments on the test groups. Some were just left in their basic 3D printed state, which others were crosslinked without AuNP or curcumin or with either 1X or 2X AuNP or curcumin. Characterization was performed in evaluating stability of each scaffold and then noting its viability, along with which types of treatment were most successful. Tasks for ascertaining viability were as follows:
The six experimental groups were:
Glover customized his own 3D printer, assembled from a CNC milling machine, with translational stages manipulated by three stepper motors. Mach3 Mill software was used in design and editing. The two most common 3D prints made during the study were a grid pattern and circles used for cell assays. Glover found that resolution was not optimum with his hardware but thought it could be finer on a higher-performance printer. Crosslinking with EDC or genipin proved to enhance both stability and durability of the 3D printed scaffolds.
The collagen-based scaffolds crosslinked with EDC exhibited ‘superb cell viability,’ although Glover pointed out that gold nanoparticles seemed to decrease success in viability somewhat. Genipin also decreased viability, which plummeted further with the addition of curcumin.
3D printing with collagen has been of great interest to researchers lately, including uses in artistic masks, bioink, and skin grafts. Read more about collagen in tissue engineering here. What do you think of this news? Let us know your thoughts! Join the discussion of this and other 3D printing topics at 3DPrintBoard.com. [Source / Images: In Situ Polymerizing Collagen for the Development of 3D Printed Tissue Engineering Scaffolds] Printing via 3DPrint.com | The Voice of 3D Printing / Additive Manufacturing https://3dprint.com March 22, 2019 at 03:27AM
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